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grna gfp t1  (Addgene inc)


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    Structured Review

    Addgene inc grna gfp t1
    Grna Gfp T1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 41 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/grna+gfp+t1/gRNA_GFP-T1+(Plasmid+%2341819)/pmc11070752-75-0-2
    Average 93 stars, based on 41 article reviews
    grna gfp t1 - by Bioz Stars, 2026-09
    93/100 stars

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    Related Articles

    Generated:

    Article Title: Variation in zygotic CRISPR/Cas9 gene editing outcomes generates novel reporter and deletion alleles at the Gdf11 locus
    Article Snippet: Oligo nucleotides were annealed, digested with BbsI (New England Biolabs) and ligated into a BbsI-digested plasmid backbone using T4 ligase (New England Biolabs). .. The backbone was generated by removing the GFP sequence from the gRNA_GFP_T1 plasmid to contain 2 BbsI sites. gRNA_GFP-T1 was a gift from George Church (Addgene plasmid # 41819; http://n2t.net/addgene:41819 ; RRID Addgene_41819) . ..

    Article Title: Variation in zygotic CRISPR/Cas9 gene editing outcomes generates novel reporter and deletion alleles at the Gdf11 locus.
    Article Snippet: Oligo nucleotides were annealed, digested with BbsI (New England Biolabs) and ligated into a BbsI-digested plasmid backbone using T4 ligase (New England Biolabs). .. The backbone was generated by removing the GFP sequence from the gRNA_GFP_T1 plasmid to contain 2 BbsI sites. gRNA_GFP-T1 was a gift from George Church (Addgene plasmid # 41819; http:// n2t.net/addgene:41819; RRID Addgene_41819)9. ..

    Sequencing:

    Article Title: Variation in zygotic CRISPR/Cas9 gene editing outcomes generates novel reporter and deletion alleles at the Gdf11 locus
    Article Snippet: Oligo nucleotides were annealed, digested with BbsI (New England Biolabs) and ligated into a BbsI-digested plasmid backbone using T4 ligase (New England Biolabs). .. The backbone was generated by removing the GFP sequence from the gRNA_GFP_T1 plasmid to contain 2 BbsI sites. gRNA_GFP-T1 was a gift from George Church (Addgene plasmid # 41819; http://n2t.net/addgene:41819 ; RRID Addgene_41819) . ..

    Article Title: Variation in zygotic CRISPR/Cas9 gene editing outcomes generates novel reporter and deletion alleles at the Gdf11 locus.
    Article Snippet: Oligo nucleotides were annealed, digested with BbsI (New England Biolabs) and ligated into a BbsI-digested plasmid backbone using T4 ligase (New England Biolabs). .. The backbone was generated by removing the GFP sequence from the gRNA_GFP_T1 plasmid to contain 2 BbsI sites. gRNA_GFP-T1 was a gift from George Church (Addgene plasmid # 41819; http:// n2t.net/addgene:41819; RRID Addgene_41819)9. ..

    Plasmid Preparation:

    Article Title: Variation in zygotic CRISPR/Cas9 gene editing outcomes generates novel reporter and deletion alleles at the Gdf11 locus
    Article Snippet: Oligo nucleotides were annealed, digested with BbsI (New England Biolabs) and ligated into a BbsI-digested plasmid backbone using T4 ligase (New England Biolabs). .. The backbone was generated by removing the GFP sequence from the gRNA_GFP_T1 plasmid to contain 2 BbsI sites. gRNA_GFP-T1 was a gift from George Church (Addgene plasmid # 41819; http://n2t.net/addgene:41819 ; RRID Addgene_41819) . ..

    Article Title: RNF43 inhibits WNT5A-driven signaling and suppresses melanoma invasion and resistance to the targeted therapy
    Article Snippet: Recombinant DNA reagent , hCas9 , Addgene #41815 , RRID: Addgene_41815 , Humanized Cas9. .. Recombinant DNA reagent , gRNA_GFP-T1 , Addgene #41819 , RRID: Addgene_41819 , gRNA expression plasmid. .. Recombinant DNA reagent , PiggyBack-Hygro; Transposase , Gifted by Bon-Kyoung Koo , RRID: Addgene_64324 , PiggyBack transposase system for stable cell lines generation.

    Article Title: Variation in zygotic CRISPR/Cas9 gene editing outcomes generates novel reporter and deletion alleles at the Gdf11 locus.
    Article Snippet: Oligo nucleotides were annealed, digested with BbsI (New England Biolabs) and ligated into a BbsI-digested plasmid backbone using T4 ligase (New England Biolabs). .. The backbone was generated by removing the GFP sequence from the gRNA_GFP_T1 plasmid to contain 2 BbsI sites. gRNA_GFP-T1 was a gift from George Church (Addgene plasmid # 41819; http:// n2t.net/addgene:41819; RRID Addgene_41819)9. ..

    Recombinant:

    Article Title: RNF43 inhibits WNT5A-driven signaling and suppresses melanoma invasion and resistance to the targeted therapy
    Article Snippet: Recombinant DNA reagent , hCas9 , Addgene #41815 , RRID: Addgene_41815 , Humanized Cas9. .. Recombinant DNA reagent , gRNA_GFP-T1 , Addgene #41819 , RRID: Addgene_41819 , gRNA expression plasmid. .. Recombinant DNA reagent , PiggyBack-Hygro; Transposase , Gifted by Bon-Kyoung Koo , RRID: Addgene_64324 , PiggyBack transposase system for stable cell lines generation.

    Expressing:

    Article Title: RNF43 inhibits WNT5A-driven signaling and suppresses melanoma invasion and resistance to the targeted therapy
    Article Snippet: Recombinant DNA reagent , hCas9 , Addgene #41815 , RRID: Addgene_41815 , Humanized Cas9. .. Recombinant DNA reagent , gRNA_GFP-T1 , Addgene #41819 , RRID: Addgene_41819 , gRNA expression plasmid. .. Recombinant DNA reagent , PiggyBack-Hygro; Transposase , Gifted by Bon-Kyoung Koo , RRID: Addgene_64324 , PiggyBack transposase system for stable cell lines generation.



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